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Dr Jackson Kung'u- Mold Specialist

Dr Jackson Kung'u- Mold Specialist

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Home | bacteria

bacteria

Testing For Sewage Contamination

Sewage contamination in building is the result of septic system backups or sewage pipe damages.  Sewage backflow in a building can damage the integrity of its structure and render it inhabitable as well. Sewage contains a range of pathogenic microorganisms like E.coli, Salmonella, Vibrio, mycobacteria, moulds, viruses and protozoa like Cryptosporidium and Giardia. Recurrent sewage leaks or spills in particular areas such as the basement of residential or commercial buildings, allow all these micro-organisms to proliferate. Exposure to these pathogens poses a serious health risk.

What Should One Do After Sewage Back-up?
Once a building gets contaminated with sewage, the most important steps to be taken are immediate removal of sewage water, disinfection of the contaminated surfaces, and prompt drying of the surfaces. Sewage remediation may require special equipment such as heavy duty vacuum cleaners. Hiring the services of remediation or restoration professionals is therefore recommended.

Documentation of contaminated materials is important. It’s difficult to clean contaminated porous materials. Therefore, any material that is porous (such as carpet, gypsum wallboard, insulation, upholstered furniture, clothing) and directly got contaminated by sewage is supposed to be discarded since the pathogenic micro-organisms have already penetrated the material.  Semi-porous material like wood furniture or pressed wood products may be thoroughly cleaned, disinfected and dried. Non-porous surfaces like metal, glass and ceramic tile floorings can be cleaned and disinfected.

coliformDetermining the effectiveness of sewage cleanup
Opinions differ as to whether microbial sampling to document effectiveness of sewage remediation is necessary. Those who think it’s not necessary argue that the primary objective of sewage remediation is to remove the sewage water and disinfecting and drying of contaminated interior surfaces. However those who support sampling recommend screening for E. coli and fecal coliforms.  E. coli (Escherichia coli in full) are found in the intestines of humans and animals and hence in fecal matter which happens to be a major component of sewage. Direct or indirect contact with E. coli contaminated water, food, air or surfaces could lead to harmful health effects. E. coli is only used as an indicator since not all strains of E. coli are pathogenic. However, E. coli strain O157:H7 is a toxin producing strain and potentially dangerous.

Even though sewage may contain many other pathogens, E. coli is the easiest to detect and identify. Hence the reason it’s used as a marker of sewage contamination.

To ensure that sewage decontamination has been effectively done, a swab test is performed on surfaces. A suspect area is identified and marked. A swab is uniformly rolled over the entire marked area (e.g. 10cm x10cm) and enclosed back into its container. Such swabs are sent to the lab at the earliest for analytical purposes. The lab would then process the swabs for detection of E. coli and other coliforms. Along with determining the presence or absence of E. coli, it is highly recommended to quantify them. Quantification gives us an idea of the bacterial load of the particular suspect area. This helps also determine the effectiveness of the disinfecting agent.

If the lab results are positive for E. coli and other coliforms, further cleaning would be required.

Filed Under: Bacteria, Coliforms Tagged With: bacteria, coliforms, e. coli, sewage

How to collect bacteria samples

Various methods can be used to collect bacteria samples. The procedure will depend on what is being sampled and the type of data required. One may may be interested in a specific bacterium, in which case the method of sampling should be suitable for the recovery of that specific organism.

Sampling from surfaces

Sampling of bacteria from surfaces is usually performed using sterile swabs. Results from this test could be as simple as presence or absense of the bacterium of interest for example E. coli or Legionella. The test could also be detailed to include the amount of the bacterium present. It’s important to know how to collect samples for these different levels of analysis. If quantification is required, it’s important to swab a known surface area, for example 100 cm square. In this case the results would be expressed as “Number of colony forming units” per unit area.

Sampling bacteria samples from the air

To sample bacteria from the air, the air is impacted on some suitable growth media.   The media to use will be determined by the type of bacteria being sampled for. If one is interested on a specific type of bacterium then a media that is selective for that bacterium should be used. To sample for the general population of bacteria in the air, then a media that can support the growth of many types of bacteria such as  tryptic soy agar (TSA) should be used. Results are given as colony forming units per cubic meter of air.

Sampling for bacteria from water

Testing water for bacteria involves collecting a water sample in a sterile container and sending it to the lab for testing. The test results may just indicate the presence/absence of the bacterium of interest or may be detailed to include the amount present in terms of colony forming units per mililitre.

Filed Under: Air Sampling, Bacteria, Bacteria Sampling Tagged With: bacteria

Listeriosis in humans and animals

Listeriosis is currently in the Canadian news, having caused a number of deaths following contamination of meat and meat products. But what is listeriosis? It’s a disease caused by species of the bacterium Listeria. There are 6 species of Listeria widely distributed in nature. Listeria monocytogenesis the causative agent of listeriosis, however other species are also pathogenic. For example, Listeria ivanovii is an animal pathogen and in rare cases cause human infection. Even though, human infection is rare, the fatality is high, about 25-30% of cases would die.

Sources of Infection

The sources of infection are food and water contaminated with soil, sewage or any other material containing Listeria. Most outbreaks are caused by eating foods from animal origin e.g. hot dogs, deli meats, cooked poultry, raw milk, cheeses, raw and smoked fish.  Unlike many other bacteria, Listeria can survive and multiply on foods being stored in the refrigerator.

Symptoms of Listeriosis in human

Listeriosis in humans is characterised by a long incubation period of up to 70 days after exposure. However, many people can be asymptomatic carriers of Listeria but few of them develop listeriosis. The symptoms of listeriosis in humans include vomiting, nausea, abdominal pain, severe headache, diarrhea or sometimes constipation with persistent fever. Pregnant women, children and old people are more likely to get listeriosis than other healthy adults. It is probable that Listeria can be transferred to unborn babies through placenta of infected pregnant women and also to newborn babies through milk.

Listeriosis “Circling disease” in animals

Listeriosis affects a wide range of animals and birds. Persistent fever, abortion and circling due to encephalitis are the common symptoms. The disease is highly sever and fatal in small ruminants. Listeria monocytogenes  can be excreted in the milk of either aborting or apparently healthy cows, ewes, and goats. Listeriosis in dogs and cats are mainly visceral and septicaemic “gastroenteritis and fever”.

How to reduce the risk of infection

To reduce the risk of infection, the following practices are recommended:

  • Thoroughly cleaning and sanitizing all surfaces used for food preparation
  • Thoroughly cleaning fruits and vegetables before eating them
  • Defrosting food in cold water or in the microwave, but never at room temperature
  • Keeping the temperature inside refrigerator under 4°C to avoid multiplication of Listeriain foods if they are already contaminated with Listeria.

 

Therapeutic treatment

Listeria monocytogenesis sensitive to penicillin (the drug of choice), cefotaxime, azithromycin and trimethoprim/sulphonamide. It is recommended to test the antimicrobial sensitivity of the isolated Listeria.

Article by: Dr. Zakaria Saleh, PhD 

References:
Health Canada, http://www.hc-sc.gc.ca/hl-vs/iyh-vsv/food-aliment/listeria-eng.php
Wikipedia, http://en.wikipedia.org/wiki/Listeriosis
The Merck Veterinary Manual, 50th Anniversary Edition

Filed Under: Bacteria, Listeria Tagged With: bacteria, Listeria

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